| 姜曲海母猪初情期前生殖器官雌激素受体mRNA表达丰度的研究 |
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| 引用本文： | 刘春玲,丁利军,丁家桐.姜曲海母猪初情期前生殖器官雌激素受体mRNA表达丰度的研究[J].扬州大学学报(农业与生命科学版),2005,26(1):33-36. |
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| 作者姓名： | 刘春玲 丁利军 丁家桐 |
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| 作者单位： | 扬州大学,动物科学与技术学院,江苏,扬州,225009;扬州大学,动物科学与技术学院,江苏,扬州,225009;扬州大学,动物科学与技术学院,江苏,扬州,225009 |
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| 基金项目： | 江苏省自然科学基金资助项目(BK2002047) |
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| 摘 要： | 采用非同位素标记原位杂交组织化学方法，检测姜曲海母猪初情期前(初生及15、30、45、60日龄)卵巢和子宫中雌激素受体(ER)mRNA的表达丰度。图文分析系统扫描结果显示：初生卵巢内ER mRNA表达不明显，15～45日龄时阳性率逐渐上升，60日龄时略有下降，阳性斑点主要集中于原始、初级、次级各级卵泡，且大卵泡阳性斑点较小、卵泡密集；子宫内ER mRNA阳性率与日龄呈正相关，且阳性斑点主要出现在子宫内膜腺体中，基质部分较少。
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| 关 键 词： | 猪 卵巢 子宫 雌激素受体mRNA 原位杂交 |
| 文章编号： | 1671-4652(2005)01-0033-04 |
| 修稿时间： | 2004年2月19日 |
ER mRNA expression in reproductive tissues of Jiangquhai sows before puberty |
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| LIU Chun-ling,DING Li-jun,DING Jia-tong.ER mRNA expression in reproductive tissues of Jiangquhai sows before puberty[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2005,26(1):33-36. |
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| Authors: | LIU Chun-ling DING Li-jun DING Jia-tong |
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| Abstract: | ER mRNA expression abundance in the reproductive issues (ovary and uterus) of Jiangauhai sows was investigated before puberty on different postnatal days (PNDs) 0, 15, 30, 45 and 60 by in stiu hybridization histochemistry. Results of image manipulation analysis system indicated that ER mRNA was almost not existed on PNDs 0,ER mRNA expression increased from PNDs 15 to 45, and declined slightly on PNDs 60. Positive stain was mainly detected in primordial follicles, primary and secondary follicles, thicker in big follicles than in small follicles. In uterus, positive intensity of ER mRNA had the significant correlations with PNDs, and ER mRNA was mainly detected in endometrium gland and less in stroma. |
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| Keywords: | swine ovary uterus ER mRNA in stiu hybridization |
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