鸭病毒性肠炎病毒致鸭胚成纤维细胞发生凋亡作用的研究

研究通过HE染色镜检发现,鸭病毒性肠炎病毒CH强毒株(DEV-CHv)可致鸭胚成纤维细胞(DEF)出现染色质颗粒化、细胞核变形等显著凋亡特征;TUNEL试验显示,接毒组细胞核内有多量棕黄色DAB显色颗粒,表明细胞出现了凋亡现象;DNA Ladder检测表明,接毒组细胞具有分子量分别为180～200bp及其整数倍的凋亡梯带电泳图谱特征;电镜观察发现,接毒组细胞具有染色质浓缩、边移,胞浆严重空泡化,细胞核严重变形,形成凋亡小体等典型凋亡特征.上述研究结果表明鸭病毒性肠炎病毒具有显著的致鸭胚成纤维细胞发生凋亡的作用.

Duck Embryo Fibroblast Apoptosis Induced by Duck Viral Enteritis Virus CH Isolate

Hematoxylin-eosin(HE) staining,terminal deoxynucleatidyl transferase mediated dUTP-biotin nick end labelling(TUNEL),DNA fragmentation analysis and electron microscopy were used to study duck embryo fibroblast(DEF) apoptosis induced by CH virulent isolate of duck enteritis virus(DEV-CHv). HE staining indicated that DEF infected with DEV-CHv showed apoptosis characterized by chromotin granulation and nucleus distortion. Results of TUNEL showed that a number of yellow-brown granules existed in nucleus of infected DEF. DNA fragmentation analysis indicated that multiples of about 180 to 200 bp oligomers appeared as a distinct DNA ladder. Electron microscopy demonstrated that infected DEF showed apoptosis characterized by chromatin condensation and margination,severe cytoplasm vacuolation, severe nucleus distortion and apoptotic bodies. The above results indicated that DEV-CHv could prominently induce apoptosis in DEF.