Fine Structural and Histochemical Study of Equine Paneth Cells |
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| Authors: | K. Takehana J. Masty M. Yamaguch A. Kobayashi O. Yamada M. Kuroda Y. S. Park K. Iwasa M. Abe |
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| Affiliation: | Veterinary Anatomy, Rakuno Gakuen University, Ebetsu, Hokkaido 069, Japan;Muscle Biology Research Laboratory. Department of Veterinary Bioscience, The Ohio State University, Columbus, Ohio 43210, U.S.A. |
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| Abstract: | Ultrastructure. lysozyme and glycoconjugate activity in duodenal Paneth cells were observed concurrently in the horse. Paneth cells were seen to uniformly line the base of the equine intestinal glands. The round secretory granules have centrally located electron densities with peripherally located electron lucent halos. Histochemically, the peripheral halo layer was positively stained for carbohydrates by the periodic acid-thiocarbohydrazide-silver protein-physical development (PA-TCH-SP-PD) method and the entire granules reacted positively to the WGA. The central core area reacted with anti-lysozyme. We identified a young (Type I) and an old (Type II) cell population in the same crypt, but we suggest that the observed populations are variations of the same cell type with the varied appearance due to aging of the secretory granules. |
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