超声波法提取黄树莓叶黄酮类物质的工艺条件优化 |
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引用本文: | 张青峰,刘咏煌,冯丽娟,张琴琴. 超声波法提取黄树莓叶黄酮类物质的工艺条件优化[J]. 安徽农学通报, 2012, 18(21): 60-62 |
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作者姓名: | 张青峰 刘咏煌 冯丽娟 张琴琴 |
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作者单位: | 遵义医学院珠海校区,广东珠海,519041;遵义医学院珠海第五附属(珠海)医院,广东珠海,519100 |
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基金项目: | 贵州省科学技术基金项目(黔科合J字[2010]2162号). |
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摘 要: | 利用超声波法提取黄树莓叶中的总黄酮,考察了超声功率、超声时间、乙醇浓度、料液比4个因素对总黄酮得率的影响,通过单因素试验和正交试验确定提取的最佳工艺条件。结果表明:黄树莓最佳提取工艺为超声波功率High档位,超声时间70min,乙醇浓度60.0%,料液比1∶25,总黄酮的得率为9.517%。该提取工艺简单、合理,可以用于测定黄树莓总黄酮的含量。
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关 键 词: | 树莓叶 总黄酮 超声波法 正交试验法 |
Optimization of Process Conditions of Total Flavonoids Extraction from Raspberry Leaves by Ul- trasonic Method |
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Affiliation: | Zhang Qingfeng et al. ( 1 Zhuhai Compus, Zunyi Medical College, Zhuhai 519041, China) |
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Abstract: | Total flavonoids was extracted from Raspberry by Ultrasonic Method. The effects of four factors including Ultrason- ic power, ultrasonic time, ethanol concentration, solid - liquid ratio on the yield of total flavonoids were investigated. The optimum technological conditions of total flavonoids extraction were confirmed through single factor test and the orthogonal test. The optimum exaction technology conditions of cloudberry were as follows:Ultrasonic power for high stall, ultrasonic time of 70 min , 60% ethanol concentrationand solid - liquid ratio of 1:25 ;the yield of total flavonoid was 9. 517%. The extraction process was simple, reasonable, and could be used for the determining the content of total flavorioids from the raspberry. |
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Keywords: | Raspberry leaves Ultrasonic method Otal flavonoids Orthogonal experiment |
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