花烛不同愈伤组织解剖结构的观察

 以花烛(Anthurium andraeanum ) ‘Jolanba’8种不同培养时期或不同激素处理的愈伤组织为材料, 通过显微观察研究了花烛离体形态发生途径和8种愈伤组织离体形态差异的解剖学特点。结果表明,花烛离体形态发生途径以间接器官(不定芽) 发生为主, 且发生于愈伤组织近表层; 同时也存在体细胞胚途径, 发生于愈伤组织表面或内部。8种愈伤组织在结节( nodule) 的类型、分生细胞的数量及分布等方面存在差异。分生细胞的数量和分布与芽的分化有较好的相关性, 愈伤组织表层分生细胞分布较多, 分化的芽也较多, 而愈伤组织内部分生细胞分布较少, 分化的芽也较少。噻二唑苯基脲( TDZ) 对分生细胞和体细胞胚有较好的诱导作用。愈伤组织的表面划痕和再次切割有利于再生芽的分化。

Observation on Anatomic Structure of Eight Kinds of Calli in Anthurium andraeanum

In vitro morphogenesis pathways and anatomic differences of eight kinds of calli in Anthurium andraeanum ‘Jolanba’at different culture stages or on the culture media supplemented with different cytokinins were researched. The results showed that the main pathwaywas indirect organgenesis, and adventitious shoot primordium derived from the subsurface layers of callus. Somatic embryogenesiswas also found both inside and on the surface of callus. Eight kinds of calli differed in types of nodule and number and distributionof meristematic cells. Number and distributing of meristematic cells was well related to shoot differentiation.Callus with more meristematic cells or outer-distributed meristematic cells could differentiate more shoots.Thidiazuron (TDZ) was showed a good effect on the induction ofmeristematic cells and somatic embryo. Nicking and making incision on callus surfaceswas helpful for shoot differentiation.