| 香蕉果实高质量总蛋白的提取方法 |
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| 引用本文： | 王卓,贾彩虹,金志强,徐碧玉.香蕉果实高质量总蛋白的提取方法[J].果树学报,2009,26(3). |
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| 作者姓名： | 王卓 贾彩虹 金志强 徐碧玉 |
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| 作者单位： | 王卓,WANG Zhuo(中国热带农业科学院热带生物技术研究所;中国热带农业科学院热带生物技术研究所);贾彩虹,金志强,徐碧玉,JIA Cai-hong,JIN Zhi-qiang,XU Bi-yu(中国热带农业科学院热带生物技术研究所)
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| 基金项目： | 国家自然科学基金，热带生物技术研究所中央级公益性科研院所基本科研业务费专项基金 |
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| 摘 要： | 比较了三氯乙酸/丙酮沉淀法、酚抽法、丙酮沉淀法3种方法对香蕉(M.AAA Group Cavendish)果实总蛋白的提取效果。结果显示酚抽法所得图谱条带完整清晰;TCA法所得图谱条带间模糊不清,存在拖尾现象;而丙酮沉淀法提取的蛋白得率不高,蛋白带谱不完整。说明酚抽法适合香蕉果实总蛋白提取。同时为满足不同激素处理及采后不同成熟度香蕉果实高质量蛋白质提取的需要,在酚抽法基础上对提取缓冲液进行了改良,获得了良好的效果。为以后研究香蕉果实蛋白质功能奠定了基础。
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| 关 键 词： | 香蕉 蛋白质提取 十二烷基磺酸钠聚丙烯酰胺凝胶电泳 酚抽法 |
Methods for total high-quality protein extraction from banana fruit |
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| WANG Zhuo,JIA Cai-hong,JIN Zhi-qiang,XU Bi-yu.Methods for total high-quality protein extraction from banana fruit[J].Journal of Fruit Science,2009,26(3). |
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| Authors: | WANG Zhuo JIA Cai-hong JIN Zhi-qiang XU Bi-yu |
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| Institution: | 1Institute of Tropical Bioscience and Biotechnology;Chinese Academy of Tropical Agricultural Science;Haikou;Hainan 571101 China;2Department of Agriculture;Hainan University;Hainan 571101 China |
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| Abstract: | Three methods of total protein extraction from banana(Musa AAA Group Cavendish)fruit,including trichloroacetic acid/acetone precipitation method,phenol extraction method and acetone precipitation method were compared in this paper.The results showed that the spectrum of proteins extracted with phenol extraction method was complete and clear;while that obtained with trichloroacetic/acetone precipitation was blurred and smearing,and using acetone precipitation resulted in low protein recovery and the protein ... |
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| Keywords: | Banana Protein extraction SDS-PAGE Phenol extraction |
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