Development of PCR primers targeting fungal nirK to study fungal denitrification in the environment |
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| Institution: | 1. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan;2. Niigata Agricultural Research Institute, Niigata 940-0826, Japan;3. Department of Bioresource Science, College of Agriculture, Ibaraki University, Ibaraki 300-0393, Japan;4. Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan;1. Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China;2. Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing, 210014, China;3. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China;4. SRUC, Crop and Soil Systems Research Group, Edinburgh, EH9 3JG, UK;1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. Department of Biological Sciences, University of Alabama, 300 Hackberry Lane, Tuscaloosa, AL, 35487, USA;2. Department of Biological Sciences, University of Southern Mississippi, 118 College Drive, Hattiesburg, MS 39406, USA;3. New College, University of Alabama, 503 6th Ave, Tuscaloosa, AL, 35487, USA;1. Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou, 310058, China;2. Changzhou Industrial Technology Research Institute of Zhejiang University, Changzhou, China;1. Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, 610213, China;2. Key Laboratory of Dryland Agriculture, Ministry of Agriculture and Rural Affairs of the People''s Republic of China, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, China;3. Environmental Stable Isotope Lab., Chinese Academy of Agricultural Sciences, Beijing, 100081, China |
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| Abstract: | Fungal denitrification in soils is receiving considerable attention as one of the dominant N2O production processes. However, because of the lack of a methodology to detect fungal denitrification-related genes, the diversity and ecological behavior of denitrifying fungi in soil remains unknown. Thus, we designed a primer set to detect the fungal nitrite reductase gene (nirK) and validated its sensitivity and specificity. Through clone library analyses, we identified congruence between phylogenies of the 18S rRNA gene and nirK of denitrifying fungal isolates obtained from the surface-fertilized cropland soil and showed that fungi belonging to Eurotiales, Hypocreales, and Sordariales were primarily responsible for N2O emissions in the soil. |
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| Keywords: | Fungal denitrification Fungal nitrite reductase |
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