杜仲梦尼夜蛾CD36家族同源基因克隆与组织分布研究

目的 揭示杜仲梦尼夜蛾CD36家族同源基因神经元膜蛋白(SNMPs)和b族清道夫受体(SRb)的序列特征和组织分布情况。 方法 设计特异性引物克隆杜仲梦尼夜蛾SNMPs和SRb基因，对这些基因编码的蛋白进行同源建模；通过荧光定量PCR分析这些基因在不同组织的表达情况。 结果 在杜仲梦尼夜蛾中鉴定得到3个CD36家族同源基因（OsonSNMP1、OsonSNMP2和OsonSRb1）；这些基因编码的蛋白具有2个跨膜区域和1个细胞外结构域。空间结构预测发现，这些蛋白的胞外结构域包含1个主要由反向平行β-折叠构成的桶状核心和1个富含疏水性氨基酸残基的α-螺旋；荧光定量PCR结果表明，OsonSNMP1、OsonSNMP2和OsonSRb1均在触角中大量表达；OsonSNMP1在雄虫触角中的表达水平显著高于雌虫触角，OsonSNMP2在雌虫触角的表达水平显著高于其他组织，OsonSRb1在雄虫触角中大量表达外，还在雌雄虫的口器中大量表达。 结论 本研究发现OsonSNMP1、OsonSNMP2和OsonSRb1编码的蛋白具有与脊椎动物CD36家族受体类似的空间结构，与嗅觉器官高度关联的表达模式表明这些基因可能在杜仲梦尼夜蛾嗅觉系统中发挥了功能，以上结果为探究杜仲梦尼夜蛾CD36家族同源基因的嗅觉功能提供了数据。

Cloning and Tissue Distribution of Homologous Genes of CD36 Family in Orthosia songi Chen et Zhang (Lepidoptera: Noctuidae)

Objective To reveal the sequence characteristics and expression profiles of CD36 homolog genes, sensory neuron membrane proteins (SNMPs) and class b scavenger receptors (SRb) in Orthosia songi Chen et Zhang. Method Specific primers were used to clone the SNMP and SRb genes. The protein encoded by these genes were constructed by homo-modeling, and the gene expression profiles were determined by quantitative fluorescent real-time PCR. Result Three CD36 homolog genes (OsonSNMP1, OsonSNMP2 and OsonSRb1) were identified from O. songi. The proteins encoded by these genes consisted of two transmembrane domains and an extracellular domain. The extracellular domain contained an antiparallel β-barrel core and a hydrophobic domain near the top. OsonSNMP1 was mainly expressed in the antennae and wings of male and female moth, and the expression level in male antennae was significantly higher than that in other tissues. OsonSNMP2 was discriminatorily expressed in male and female antennae and female wings, and its expression level in female antennae was significantly higher than that in other tissues. OsonSRb1 were specifically expressed in the antennae and mouthparts of male and female. Conclusion The proteins encoded by OsonSNMP1, OsonSNMP2 and OsonSRb1 have similar structures with CD36 receptors in vertebrates. Their expression profiles highly associated with olfactory organs indicate the potential roles in olfactory systems. These results provide experimental data for further study on the olfactory function of CD36 homolog genes in Orthosia songi.