Determination of the optimal soil sample size to accurately characterise nematode communities in soil |
| |
| Affiliation: | 1. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China;2. University of the Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China;3. Ecological Sciences, The James Hutton Institute, Dundee, DD2 5DA, Scotland, UK;4. Department of Animal and Plant Sciences, The University of Sheffield, Sheffield S10 2TN, UK;5. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
| |
| Abstract: | Analysis of nematode communities is a potential biological proxy to monitor soil health. Traditionally, nematodes are extracted from 200 g of soil and then identified based on morphology. However, the optimal soil sample size to accurately characterise nematode communities using molecular methods is unknown. Using a combined relative real-time PCR and T-RFLP approach we analysed nematode communities extracted from triplicate samples ranging from 1 to 200 g soil. Our data indicated that for molecular based analyses, soil sample sizes <200 g of soil do not accurately represent the abundance of nematodes and <100 g samples are less likely to reflect the true community composition. Thus characterisation of nematode communities from low sample sizes may not be robust. |
| |
| Keywords: | Assemblage Community Nematode Relative real-time PCR Soil quality |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
