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| BtR05的诱变育种及其对朱砂叶螨的毒力测定 | |
| 引用本文： | 赵晓燕,王贻莲,魏艳丽,张广志,李纪顺,杨合同.BtR05的诱变育种及其对朱砂叶螨的毒力测定[J].福建农林大学学报(自然科学版),2009,38(5). |
| 作者姓名： | 赵晓燕 王贻莲 魏艳丽 张广志 李纪顺 杨合同 |
| 作者单位： | 1. 山东省科学院中日友好生物技术研究中心,山东,济南,250014 2. 山东省科学院生物研究所,山东,济南,250014 3. 山东省科学院中日友好生物技术研究中心,山东,济南,250014;山东省科学院生物研究所,山东,济南,250014 |
| 基金项目： | 山东省科技攻关项目，山东省自然科学基金资助项目 |
| 摘 要： | 以BtR05为原始菌株,通过紫外诱变,获得一株对朱砂叶螨成螨有较高毒力的突变株63号.63号菌株的生长速度快,繁殖力强,LB摇瓶培养48 h后显微计数为4.88×109cfu.mL-1.63号发酵液的杀螨活性是原始菌株的1.33倍.通过斜面传代培养,该菌株能稳定遗传10代. |
| 关 键 词： | 苏云金芽孢杆菌 紫外诱变 朱砂叶螨 毒力测定 遗传稳定性 |
Selection of BtR05 by UV mutation and determination of its toxicity to Tetranychus cinnabarinus |
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| ZHAO Xiao-yan,WANG Yi-lian,WEI Yan-li,ZHANG Guang-zhi,LI Ji-shun,YANG He-tong.Selection of BtR05 by UV mutation and determination of its toxicity to Tetranychus cinnabarinus[J].Journal of Fujian Agricultural and Forestry University,2009,38(5). | |
| Authors: | ZHAO Xiao-yan WANG Yi-lian WEI Yan-li ZHANG Guang-zhi LI Ji-shun YANG He-tong |
| Institution: | ZHAO Xiao-yan1,WANG Yi-lian1,WEI Yan-li2,ZHANG Guang-zhi1,LI Ji-shun1,YANG He-tong1,2(1.China-Japan Friendship Biotechnology Research Center,2.Biology Research Institute,Shandong Academy of Sciences,Jinan,Shandong 250014,China) |
| Abstract: | By using BtR05 as the original strain, mutant strain 63 having higher toxicity to adult mites of Tetranychus cinnabarinus was obtained by UV mutation.The strain grew faster and bred stronger than parental strain, its microscope counting number was 4.88 × 10~9 cfu · mL~(-1) after shaking cultivation 48 h in LB liquid. The toxicity to adult mites of its fermentative broth was 1.33 times as much as BtR05. The strain could transmit 10 generations steadily through slant subculture. |
| Keywords: | Bacillus thruringiensis UV mutation Tetranychus cinnabarinus toxicity determination transmit stability |
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