Characterization of bovine coronavirus isolates/from eight different states in the USA. |
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| Authors: | S Kapil K L Richardson T R Maag S M Goyal |
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| Institution: | Department of Diagnostic Medicine-Pathobiology College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA. kapil@vet.ksu.edu |
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| Abstract: | Bovine coronavirus isolates from eight different states of the USA were compared for their antigenic properties and susceptibility to hygromycin B. Antigenic differences were observed among the isolates in a one-way hemagglutination-inhibition (HI) test using a polyclonal antiserum against the Mebus bovine coronavirus isolate. Differences were observed on isoelectric focusing among viral proteins with isoelectric points between 4.45-4.65. Most of the BCV isolates were susceptible to hygromycin B (0.5 mM) whereas a few hygromycin B resistant isolates were also found. |
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