Detection of Tobamoviruses from Soils by Non-precoated Indirect ELISA |
| |
| Authors: | Shigeharu TAKEUCHI Yasufumi HIKICHI Yoichi KAWADA Tetsuro OKUNO |
| |
| Institution: | (1) Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University, Nankoku 783-8502, Japan, JP;(2) Laboratory of Plant Pathology, Kochi Agricultural Research Center, Nankoku 783-0023, Japan, JP |
| |
| Abstract: | A method for detecting tobamoviruses from field soils was developed using non-precoated indirect enzyme-linked immunosorbent
assay (Id-ELISA). Absorbance values in Id-ELISA were relatively low after directly applying Pepper mild mottle virus (PMMoV)-infested soil extract. However, heat treating the soil extract before application greatly enhanced the absorbance
values. The heat treatment was essential for the Id-ELISA detection of tobamoviruses from infested soil, although the efficiency
of virus recovery varied depending on the properties of soil. The number of local lesions in the infectivity assay was consistent
with the absorbance values in Id-ELISA. Moreover, the absorbance values in Id-ELISA were correlated with the incidence of
soil transmission of PMMoV. Thus, Id-ELISA combined with heat treatment is a practical technique for the diagnosis of infestation
with Tobamovirus in field soils, Gray Lowland soil and Sand-dune Regosol.
Received 4 October 1999/ Accepted in revised form 9 December 1999 |
| |
| Keywords: | : Tobamovirus green pepper infested soil indirect-ELISA detection |
| 本文献已被 SpringerLink 等数据库收录！ |
|
