| 鹅副粘病毒GX1株的HN和F蛋白基因的克隆和序列分析 |
| |
| 引用本文： | 易春华,潘杰,付薇,颜健华,徐贤坤,熊毅.鹅副粘病毒GX1株的HN和F蛋白基因的克隆和序列分析[J].农业科学与技术,2009,10(4):75-78. |
| |
| 作者姓名： | 易春华 潘杰 付薇 颜健华 徐贤坤 熊毅 |
| |
| 作者单位： | 易春华,潘杰(广西大学动物科学技术学院,广西南宁,530005);付薇,颜健华,徐贤坤,熊毅(广西壮族自治区动物疫病预防控制中心,广西南宁,530001) |
| |
| 基金项目： | the Development Program for Guangxi Science and Technology(0719004-3G).广西科技攻关项目 |
| |
| 摘 要： | 1材料与方法
1．1病毒来源鹅副粘病毒分离株GX1株由广西壮族自治区动物疫病预防控制中心实验室分离保存。
1．2试剂及仪器各种PCR反应试剂、PMD-18T载体、胶回收试剂盒、限制性内切酶等均购自宝生物工程（大连）公司。仪器：恒温孵化箱、PCR反应仪、超速离心机、移液器等。
|
| 关 键 词： | 鹅副粘病毒 F蛋白基因 序列分析 广西壮族自治区 病毒分离株 PCR反应 克隆 动物疫病预防 |
Cloning and Sequence Analysis of HN and F Protein Genes from a Strain of Goose Paramyxovirus |
| |
| YI Chun-hua,PAN Jie,FU Wei,YAN Jian-hua,XU Xian-kun,XIONG Yi.Cloning and Sequence Analysis of HN and F Protein Genes from a Strain of Goose Paramyxovirus[J].Agricultural Science ＆ Technology,2009,10(4):75-78. |
| |
| Authors: | YI Chun-hua PAN Jie FU Wei YAN Jian-hua XU Xian-kun XIONG Yi |
| |
| Institution: | 1. College of Animal Science and Technology, Guangxi University, Nanning 530005; 2. Guangxi Provincial Center for Animal Disease Control and Prevention, Nanning 530001) |
| |
| Abstract: | Objective] The study was to clone HN and F genes from GX1 strain of goose paramyxovirus and analyze their sequences. Method] According to the full nucleotide sequence of GPV-SF02 strain of goose paramyxovirus, two pairs of pdmers were designed to amplify the HN and F genes from GX1 strain of goose paramyxovirus isolated from diseased goose in Guangxi Zhuang Autonomous Region; the amplified products were ligated into pMD18-T vector and sequenced. Result ] HN and F genes of this strain tested were 1 716 and 1 662 bp in full nucleotide length, respectively; both showed the homologues of about 97.3% with GPV- SF02 strain, of 80.3% -97.5% with strains LaSota, F48E9 and JS, of just 84.8% with Miyadera strain. Conclusion] The results show that isolated strain BX1 matches to virulent APMV-1 strain, belonging to genotype Ⅶ of APMV-1 strain. |
| |
| Keywords: | Goose paramyxovirus HN protein gene F protein gene Cloning Sequence analysis |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
