L-茶氨酸对小鼠肠道形态结构及游离氨基酸的影响

将50只KM雄性小鼠按每组10只随机分为正常组、谷氨酰胺组及L-茶氨酸低、中、高剂量组,连续灌喂28 d,探究L-茶氨酸对小鼠肠道形态结构及游离氨基酸的影响。结果表明,与正常组相比,小鼠肠道绒毛高度随L-茶氨酸剂量的增大而增加,各剂量组的十二指肠绒毛高度和中、高剂量组的空肠绒毛高度及其绒毛高度与隐窝深度的比值均达显著水平(P<0.05),血清游离氨基酸含量及中、高剂量组的尿素氮含量显著降低(P<0.05),高剂量组的碱性磷酸酶、总蛋白含量显著升高(P<0.05);L-茶氨酸对不同肠道游离氨基酸含量的影响随剂量的增大而升高,其中,十二指肠中的各茶氨酸剂量组的全部必需氨基酸和大部分非必需氨基酸显著升高(P<0.05),且高剂量组的必需氨基酸含量显著高于低剂量组(P<0.05);空肠中的低剂量组的必需氨基酸Thr、Lys、Ile及中、高剂量组的全部必需氨基酸含量显著升高,且中、高剂量组中除Cit外的其他氨基酸含量均显著高于低剂量组(P<0.05);回肠中的低、中、高剂量组的全部必需氨基酸及大部分非必需氨基酸含量显著降低,且中、高剂量组中除GABA外的其他氨基酸含量显著高于低剂量组(P<0.05)。结果表明,L-茶氨酸可通过增加肠道绒毛高度、提高绒毛高度与隐窝深度比值来改善小鼠肠道形态结构,同时可促进十二指肠、空肠对氨基酸的吸收利用。

Effects of L-theanine on Intestinal Morphology and Free Amino Acids in Mice

Fifty KM male mice were randomly divided into the normal, glutamine and low, medium and high dose L- theanine treatment groups with 10 mice/group. The mice were continuously fed for 28 days to investigate the effects of L-theanine on intestinal morphology and free amino acids. The results show that compared with the normal group, the intestinal villus height increased with the increase of L-theanine dosage. The duodenal villus height in each L-theanine groups, the jejunum villus height and villus height/crypt depth in the middle and high dose L-theanine groups were significantly increased (P<0.05). The levels of free amino acids in serum, urea nitrogen in high- and