外源一氧化氮对低温胁迫下茶树幼苗生理特性的影响

为探究外源一氧化氮(Nitric oxide,NO)供体硝普钠(Sodium nitroprusside,SNP)对低温胁迫下茶树幼苗的缓解作用,采用人工气候室模拟低温胁迫试验,研究了叶面喷施不同浓度SNP对低温胁迫下两年生碧香早茶苗叶片膜脂过氧化程度、渗透调节物质含量及抗氧化酶活性的影响。结果表明,适宜SNP浓度可降低低温胁迫下茶树叶片相对电导率,抑制丙二醛含量的升高,促进脯氨酸、可溶性蛋白和可溶性糖的大量积累,提高超氧化物歧化酶、过氧化氢酶活性,从而缓解低温胁迫对茶树叶片的伤害。试验结果表明,喷施浓度为200μmol·L^-1的SNP效果最佳。

Effects of Exogenous Nitric Oxide on Physiological Characteristics of Tea Plants Under Cold Stress

In order to investigate the alleviation effect of exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) on tea rooted cuttings under cold stress, artificial climate chamber was used to simulate cold stress on tea rooted cuttings. The effects of SNP on membrane lipid peroxidation, osmotic adjustment substance and antioxidant activity of biennial ‘Bixiangzao’ rooted cuttings under cold stress were studied by foliar spray of SNP. The results show that suitable SNP concentration treatment could reduce the relative conductivity of tea leaves, inhibit the elevated malondialdehyde content, promote the accumulation of proline, soluble protein and sugar, and increase the superoxide dismutase, catalase activities, thereby alleviate the damage of cold stress on tea leaves. In addition, the optimal concentration of SNP was 200 μmol L^-1.