桦褐孔菌发酵菌粉成分分析

对桦褐孔菌(lnonotus obliquus)发酵菌粉中的各种成分进行了较全面的分析.结果表明发酵菌粉中营养成分较丰富,其中粗蛋白、总氨基酸含量分别为25.73％和15.18％.菌粉中不饱和脂肪酸是优势脂肪酸,以亚油酸和油酸为主,还含有丰富的甾醇、酚类物质和多种矿质元素.用桦褐孔菌发酵菌粉提取粗多糖,得率为28.55％、多糖的分子量主要集中在2～40 kDa,单糖组成为阿拉伯糖、甘露糖、葡萄糖、半乳糖,摩尔比为:1.3:1.0:20.4:2.1,多糖产品中含有酸性多糖,糖苷键主要是a型.

Compositional Analysis of Spray-dried Inonotus obliquus Cultures

Inonotus obliquus was grown for 150 h in 1500-L fermenters containing 800 L medium(pH 4.0) consisting of 2% glucose,1% corn powder,1.5% wheat bran extract(15 g wheat bran was suspended in 100 mL deionized water,heated at 90 ℃ for 0.5 h,centrifuged,and 10 mL supernatant diluted 1∶9 with deionized water),0.3% peptone and 0.1% MgSO4.Compositional analysis of the spray-dried submerged cultures(culture fluid and fungal mycelium) revealed the material to contain crude protein(25.73%),amino acids(15.18%),fatty acids(78.02% unsaturated consisting mainly of linoleic and oleic acids),sterols,phenols and several mineral elements.Water extraction and ethanol precipitation yielded 28.55% polysaccharide(some of which was acidic polysaccharide),with a molecular weight distribution concentrated mainly within the 2~40 kDa range.The polysaccharide material was composed of arabinose,mannose,glucose and galactose in an overall molar ratio of 1.3∶1.0∶20.4∶2.1 and linked together mainly by α-type glycosidic bonds.