In vitro induction of tetraploids in Phlox subulata L. |
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Authors: | Zhihong Zhang Hongyan Dai Min Xiao Xin Liu |
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Institution: | (1) College of Horticulture, Shenyang Agricultural University, Dongling Road 120, Shenyang, Liaoning, 110161, P.R. China |
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Abstract: | Tetraploid plants of Phlox subulata L. were induced successfully by treating shoot tips in vitro with colchicine. Shoot tips excised from in vitro shoots were
treated with four different concentrations of colchicine (0.005, 0.01, 0.02, 0.04%) in solid MS medium supplemented with 4.54 μM
TDZ and 0.49 μM IBA for 10, 20 or 30 days, respectively. The survival rates of shoots tips were affected by the concentration
of colchicine and the duration of treatment. High concentration and longer duration reduced survival of the shoot tips, but
the effect of duration of colchicine was more than that of concentration. Tetraploid plants were obtained in all of the treatments,
but the percentages of tetraploids varied among different treatments, from 25.0% to 75.0%. The most efficient condition for
inducing tetraploids was to treat shoot tips with 0.005% colchicine for 20 days, with 30.0% survival rate of shoot tips and
6 tetraploid plants out of 10 plants examined. The rooted tetraploid plants were transplanted successfully in a solar greenhouse.
Under the same growing condition, significant varieties in flower bud and flower sizes were detected between 2x and 4x plants. The flower diameters of tetraploid and diploid plants were 2.91 cm and 2.24 cm, respectively. |
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Keywords: | Phlox subulata L Tetraploid Induction Colchicine Shoot tip Flower diameter |
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