基于GC-MS技术对盐胁迫小麦小分子代谢物的研究

采用GC-MS技术对不同浓度盐胁迫处理的小麦(辽春17号)叶片进行分析,探究其代谢物的差异。结果表明,浓度为200、250 mmol·L^-1和300 mmol·L^-1的盐胁迫处理组与对照组的小分子代谢物呈现差异性,表现为显著差异的代谢物16个,包括氨基酸类代谢物7个、糖类代谢物3个、有机酸类代谢物5个、其他物质1个;极显著差异的代谢物7个,包括糖类代谢物2个、有机酸类代谢物4个、其他物质1个。发现了相关代谢通路11条,影响最大的为甘氨酸,丝氨酸和苏氨酸代谢通路,影响因子为0.53。

Study on Small Molecule Metabolites of Salt-stressed Wheat Based on GC-MS Technology

In this paper,differences in metabolites of leaves in wheat variety Liaochun 17 under different salt stress concentrations were studied based on GC-MS analysis.The results showed that there were significant difference of small molecule metabolites between the salt stress treatment with 200,250 and 300 mmol·L^-1 concerntraions and the control.There were 16 metabolites with significant differences,including 7 amino acids metabolites,3 sugars metabolites,5 organic acids metabolites and 1 other substance while there were 7 extremely significant metabolites,including 2 carbohydrate metabolites,4 organic acid metabolites and 1 other substance.Meanwhile,eleven related metabolic pathways were found,and the most influential ones were pathways of glycine,serine and threonine which the influencing factors were 0.53.