| 棉花杂交种种子纯度的SSR分子标记鉴定方法 |
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| 引用本文： | 金彦龙,李婧慧,乔艳清,赵然然,张新宇,孙杰,薛飞.棉花杂交种种子纯度的SSR分子标记鉴定方法[J].种子,2020(3):34-38. |
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| 作者姓名： | 金彦龙 李婧慧 乔艳清 赵然然 张新宇 孙杰 薛飞 |
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| 作者单位： | 石河子大学农学院 |
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| 基金项目： | 兵团现代农业科技攻关与成果转化计划(2015 AC 007);石河子大学新品种培育专项(YZZX 201704)。 |
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| 摘 要： | 种子的纯度是影响杂交棉发挥其杂种优势的重要原因。本研究基于不同棉花品系之间的遗传变异,筛选出杂交种亲本间具有多态性,且为共显性的SSR核心引物,用于杂交种种子纯度鉴定。通过统计每个检测引物扩增带型的差异或一致性,综合得出杂交种的种子纯度。利用该方法,对4个棉花杂交种种子的纯度进行SSR分子标记鉴定,为棉花杂交种纯度鉴定提供参考。
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| 关 键 词： | 棉花 杂交种 SSR标记 纯度鉴定 |
Identification Seed Purity of Cotton Hybrids by SSR Molecular Markers |
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| JIN Yanlong,LI Jinghui,QIAO Yanqing,ZHAO Ranran,ZHANG Xinyu,SUN Jie,XUE Fei.Identification Seed Purity of Cotton Hybrids by SSR Molecular Markers[J].Seed,2020(3):34-38. |
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| Authors: | JIN Yanlong LI Jinghui QIAO Yanqing ZHAO Ranran ZHANG Xinyu SUN Jie XUE Fei |
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| Institution: | (College of Agronomy,Shihezi University,The Key Laboratory of Oasis Eco-agriculture,Xinjiang Production and Construction Group,Shihezi Xinjiang 832003,China) |
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| Abstract: | Seed purity is an important factor affecting the heterosis of hybrid cotton.In this study,SSR molecular markers were used to rapidly identify the purity of four kinds of cotton hybrids.And,the SSR core primers with polymorphism and codominance among parents were screened out.Based on the effects of genetic variation between genes on the purity detection of hybrid cotton varieties,the number of primers suitable for purity identification of hybrid cotton varieties and the optimal detection particle number were confirmed,which provided a convenient and standard method for the fast identification of hybrids and recogniation of authenticity for breeders and markets in the future. |
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| Keywords: | cotton hybrid seed SSR markers purity identification |
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