十里香茶离体培养及再生体系研究

为了保护珍贵的十里香茶树资源,掌握利于十里香茶的最优组培条件,本研究对十里香茶组培过程中外植体类型、消毒时间、培养基添加物及培养条件进行了筛选。结果表明,采用轻微木质化的带腋芽茎段经升汞消毒15 min,接种后暗培养5 d,MS+0.2 mg·L^-1 NAA培养基中添加3 mg·L^-1 PVP+2000 mg·L^-1 Vc或者6 mg·L^-1 PVP+3000 mg·L^-1 AC,可在低污染率的前提下将褐化率降为10%左右,一定程度上解决了茶树组培中褐化率高的难题;采用1/8 MS+1 mg·L^-1 IBA培养基有利于外植体生根且增殖迅速。本研究建立了十里香茶的无菌苗离体培养及再生体系,可通过组培手段对其进行大量繁殖。

Study on in Vitro Culture and Regeneration System of Camellia sinensis cv.Kunming Shilixiang

In order to protect the precious resources of Camellia sinensis cv.Shilixiang and investigate the optimal tissue culture conditions for this tea variety,the tissue culture-related factors inculding the explants type,disinfection time,medium additives and culture conditions were screened out during tissue culture of Shilixiang.The results showed that the slightly lignified stem with axillary buds were disinfected with mercury bichloride for 15 minutes and then dark cultured for 5 days after inoculation in the MS medium containing 0.2 mg·L^-1 NAA,3 mg·L^-1 PVP and 2000 mg·L^-1 Vc or 0.2 mg·L^-1 NAA,6 mg·L^-1 PVP and 3000 mg·L^-1 AC could reduce the browning rate to about 10%on the premise of low contamination rate,which the problem of high browning rate in tea tissue culture was solved to some extent.Additionally,the 1/8 MS medium added 1 mg·L^-1 IBA was beneficial to the rooting and rapid proliferation of tea explants.In this study,an in Vitro culture and regeneration system for sterile seedlings of tea variety Shilixiang was established,which provided a technique for the mass propagation of Shilixiang based on tissue culture.