| 苦丁茶不同提取物抗氧化活性比较及其GC-MS分析 |
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| 引用本文： | 朱科学,刀春丽,顾文亮,徐飞,谭乐和.苦丁茶不同提取物抗氧化活性比较及其GC-MS分析[J].热带作物学报,2020,41(3):579-585. |
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| 作者姓名： | 朱科学 刀春丽 顾文亮 徐飞 谭乐和 |
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| 作者单位： | 1. 中国热带农业科学院香料饮料研究所,海南万宁 5715332. 海南省热带香料饮料作物工程技术研究中心,海南万宁 5715333. 云南农业大学热带作物学院,云南普洱 665000 |
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| 基金项目： | 中国热带农业科学院基本科研业务费专项资金(1630142017019) |
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| 摘 要： | 利用气相色谱-质谱联用技术（GC-MS）,对苦丁茶超声辅助不同有机溶剂（乙醇、乙酸乙酯、石油醚）提取物及超临界CO2萃取物的体外抗氧化活性（DPPH自由基清除能力、羟基自由基清除活性、超氧阴离子自由基清除活性和ABTS +自由基清除能力）进行比较分析。结果表明,苦丁茶不同提取物DPPH清除能力和超氧阴离子自由基清除活性依次为：超声-乙醇提取物>超临界CO2萃取物>超声-乙酸乙酯提取物>超声-石油醚提取物,羟基自由基和ABTS +自由基清除能力依次为：超声-乙醇提取物>超声-乙酸乙酯提取物>超临界CO2萃取物>超声-石油醚提取物。GC-MS结果显示,苦丁茶不同提取物中主要有5类物质,共有49种化学成分,7种共有成分。
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| 关 键 词： | 苦丁茶 提取物 超临界CO2 体外抗氧化活性 气相色谱-质谱 |
| 收稿时间： | 2019-05-29 |
Antioxidant Activities and GC-MS Analysis of Ilex kudingcha C.J. Tseng Extracts by Different Extraction Methods |
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| ZHU Kexue,DAO Chunli,GU Wenliang,XU Fei,TAN Lehe.Antioxidant Activities and GC-MS Analysis of Ilex kudingcha C.J. Tseng Extracts by Different Extraction Methods[J].Chinese Journal of Tropical Crops,2020,41(3):579-585. |
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| Authors: | ZHU Kexue DAO Chunli GU Wenliang XU Fei TAN Lehe |
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| Institution: | 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China2. Hainan Provincial Engineering Research Center for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China3. College of Tropical Crop Science, Yunnan Agricultural University, Pu’er, Yunnan 665000, China; |
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| Abstract: | The present study was aimed to compare the effects of the extracts of Ilex kudingcha C.J. Tseng with different organic solvents (ethanol, ethyl acetate, petroleum ether) assisted by ultrasound and supercritical CO2 extraction against oxidation activities (DPPH?) scavenging activity, hydroxyl radical (?OH) elimination activity, superoxide anion free radical (O2 -?) scavenging activity and ABTS +? radical elimination ability), and component analysis by gas chromatography-mass spectrometry (GC-MS). The DPPH? and O2 -? scavenging abilities were as follows: ultrasound-ethanol extract > supercritical CO2 extract > ultrasound-ethyl acetate > ultrasound-petroleum ether extract. The OH? and ABTS +? radical scavenging abilities were as follows: ultrasound-ethanol extract > ultrasound-ethyl acetate extract > supercritical CO2 extract > ultrasound-petroleum ether extract. GC-MS analysis showed that there were five main types of substances in the four different extraction methods, a total of 50 chemical components and seven common components. |
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| Keywords: | Ilex kudingcha C J Tseng extracts supercritical CO2 in vitro antioxidant activity GC-MS |
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