| 基于COI基因的橡胶树六点始叶螨遗传多样性分析 |
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| 引用本文： | 聂跃,卢塘飞,陈俊谕,张方平,牛黎明,符悦冠.基于COI基因的橡胶树六点始叶螨遗传多样性分析[J].热带作物学报,2020,41(4):773-778. |
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| 作者姓名： | 聂跃 卢塘飞 陈俊谕 张方平 牛黎明 符悦冠 |
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| 作者单位： | 1. 海南大学植物保护学院,海南海口 5702282. 中国热带农业科学院环境与植物保护研究所,海南海口 5711013. 农业农村部热带作物有害生物综合治理重点实验,海南海口 571101 |
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| 基金项目： | 天然橡胶产业技术体系(CARS-33-BC2);中国热带农业科学院基本科研业务费(1630042017009);“一带一路”热带项目(BARTP-08) |
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| 摘 要： | 六点始叶螨是橡胶树上的重要害螨之一,具有分布范围广、危害重等特点。为了研究其遗传多样性和遗传分化,本研究利用线粒体COI（mitochondrial cytochrome oxidase subunit I）基因序列对采自海南、云南、越南河内等地区橡胶树上的11个不同地理种群的165头样本进行了种群的遗传多样性分析。获得的六点始叶螨线粒体COI基因片段长度均为658 bp,分析发现可变位点30个,其中简约信息点15个,单变异位点15个,单倍型15种;种群间FST值为0.34698,Nm值为0.47,GST值为0.19660,表明11个种群的六点始叶螨存在一定的遗传分化。
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| 关 键 词： | 六点始叶螨 COI 地理种群 遗传分化 |
| 收稿时间： | 2019-08-01 |
Geographical Population Genetic Differentiation Analysis of Eotetranychus sexmaculatus in Rubber Tree Based on Mitochondrial COI Gene Sequences |
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| NIE Yue,LU Tangfei,CHEN Junyu,ZHANG Fangping,NIU Liming,FU Yueguan.Geographical Population Genetic Differentiation Analysis of Eotetranychus sexmaculatus in Rubber Tree Based on Mitochondrial COI Gene Sequences[J].Chinese Journal of Tropical Crops,2020,41(4):773-778. |
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| Authors: | NIE Yue LU Tangfei CHEN Junyu ZHANG Fangping NIU Liming FU Yueguan |
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| Institution: | 1. College of Plant Protection, Hainan University, Haikou, Hainan 570228, China2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China3. Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China |
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| Abstract: | Eotetranychus sexmaculatus (Riley) is one of the most important pests of rubber trees, with a wide geographical distribution and heavy infestation. In order to study its genetic diversity and genetic differentiation, the mitochondrial COI gene sequence was used to analyze the genetic structure of 165 individuals from 11 different geographical populations collected from rubber trees in Hainan, Yunnan and Hanoi (Vietnam). The obtained mitochondrial COI gene fragment of the six-pointed spider mites was 658 bp in length. There were 30 variable sites, including 15 simple information points, 15 single mutation sites, and 15 haplotypes. The FST value between populations was 0.34698, the Nm value was 0.47, and the GST value was 0.19660, indicating that there was genetic differentiation in the 11 populations of E. sexmaculatus (Riley). |
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| Keywords: | Eotetranychus sexmaculatus (Riley) COI geographic population genetic differentiation |
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