| 液相色谱—串联质谱法测定牛肉中磺胺类抗生素 |
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| 引用本文： | 李谦,刘正华,李拥军.液相色谱—串联质谱法测定牛肉中磺胺类抗生素[J].现代农业科技,2012(2):345-346. |
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| 作者姓名： | 李谦 刘正华 李拥军 |
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| 作者单位： | 广东省中山市小榄镇农业服务中心;中山市农产品质量监督检验所 |
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| 基金项目： | 国家自然科学基金项目(30670380);农业部生态农业重点开放实验室开放课题(2009k07);农业科技攻关项目(20114A199) |
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| 摘 要： | 采用液相色谱—串联质谱法检测牛肉中的7种磺胺类药物残留。样品经乙腈提取,正己烷脱脂净化,液相色谱—串联质谱法检测。结果表明:7种磺胺类药物均具有良好的线性关系,相关系数不低于0.998,高、中、低3档加标回收率为92.5%~100.6%,相对标准偏差为2.0%~4.5%,检出限为1.0~4.5μg/L。该方法具有检测结果准确可靠、简便快速等优点。
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| 关 键 词： | 牛肉 磺胺类药物 残留 液相色谱—串联质谱法 |
Determination of Sulfonamide in Beef by High Performance Liquid Chromatography-tandem Mass Spectrometry |
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| Authors: | LI Qian LIU Zheng-hua LI Yong-jun |
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| Institution: | 1Agricultural Service Center of Xiaolan Town in Zhongshan City of Guangdong Province,Zhongshan Guangdong 528415;2 Zhongshan Quality Supervision & Inspection Institute of Agricultural Products) |
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| Abstract: | A HPLC-MS/MS method for determination of 7 sulfonamides residues in beef was made in the paper.The sample was extracted with acetonitrile and anhydrous sodium sulfate,purified and defatted with n-hexane,then detected by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The results showed that calibration curves for 7 sulfonamides residues all had good linear relationships with correlation coefficients not less than 0.998.The spiked recoveries at three concentrations were ranged from 92.5% to 100.6%,with relative standard deviations of 2.0%~4.5%.The limits of detection were in the range of 1.0~4.5 μg/L.It was an effective method which fitted for the sulfonamides residues analysis and had high accuracy,precision and sensitivity. |
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| Keywords: | beef sulfonamides residue HPLC-MS/MS |
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