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| 云南茶树种质的RAPD研究 | |
| 引用本文： | 邵宛芳,庞瑞华,王平盛,许玫,段红星,张亚萍,李家华. 云南茶树种质的RAPD研究[J]. 中国农业科学, 2003, 36(12): 1582-1587 |
| 作者姓名： | 邵宛芳 庞瑞华 王平盛 许玫 段红星 张亚萍 李家华 |
| 作者单位： | 1. 云南农业大学茶学系,昆明,650201 2. 河南省信阳师范学院生物系,信阳,464000 3. 云南省农业科学研究院茶叶研究所,勐海,666201 |
| 基金项目： | 云南省自然科学基金资助项目 ( 2 0 0 0C0 0 50M ) |
| 摘 要： | 利用RAPD技术对云南省主要产茶区具有代表性的茶树种质的遗传多样性进行研究。从 40个随机引物中 ,筛选出 8个扩增效果良好的引物 ,用其共扩增出 95条DNA带 ,其中 90条为多态性带 ,占 94.7% ,平均每个引物扩增的DNA带数为 11.50条。对 45个茶树种质间的亲缘关系进行UPGMA聚类分析 ,结果表明 ,当以欧氏距离为 5.0来划分时 ,试验材料可分为 7个组 ,其中 5个复合组、2个独立组 ,基本上与茶系分类水平相吻合。在 5个复合组内 ,与种级分类水平相吻合 ,而 2个独立组具有独特的种质特性 |
| 关 键 词： | 茶树 云南 RAPD 遗传多样性 |
| 修稿时间： | 2002-09-05 |
RAPD Analysis of Tea Trees in Yunnan |
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| SHAO Wan-fang ,PANG Rui-hua ,WANG Ping-sheng ,XU Mei ,DUAN Hong-xing ,ZHANG Ya-ping ,LI Jia-hua. RAPD Analysis of Tea Trees in Yunnan[J]. Scientia Agricultura Sinica, 2003, 36(12): 1582-1587 | |
| Authors: | SHAO Wan-fang PANG Rui-hua WANG Ping-sheng XU Mei DUAN Hong-xing ZHANG Ya-ping LI Jia-hua |
| Affiliation: | SHAO Wan-fang 1,PANG Rui-hua 2,WANG Ping-sheng 3,XU Mei 3,DUAN Hong-xing 1,ZHANG Ya-ping 1,LI Jia-hua 1 |
| Abstract: | RAPD assessment on genetic variations of 45 germplasm s in teas was carried out. 8 primers selected from 40 random primers were used to amplify 45 tea samples, and a total of 95 DNA bands were amplified, among which 90 (94.7%) were polymorphic. The average number of DNA bands amplified by each p rimer was 11.50. UPGMA cluster analysis of 95 DNA bands amplified by 8 primers w as made. The results showed that all the materials tested could be classified in to 7 groups including 5 complex groups and 2 simple groups, which was basically identical with morphological classification, besides these, there were some spec iation in 2 simple groups. |
| Keywords: | Tea trees Yunnan RAPD Genetic diversity |
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