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| 应用聚合酶链式反应(PCR)技术诊断仔猪水肿病 | |
| 引用本文： | 杨莉,吴位珩,杨茂生,贾慧珺,杨胜坤,杨相策.应用聚合酶链式反应(PCR)技术诊断仔猪水肿病[J].贵州农业科学,2009,37(6). |
| 作者姓名： | 杨莉 吴位珩 杨茂生 贾慧珺 杨胜坤 杨相策 |
| 作者单位： | 1. 贵州省畜牧兽医研究所,贵州,贵阳,550005 2. 贵阳市花溪区兽医防疫站,贵州,贵阳,550005 3. 贵州省黔东南州三穗县八恭寨人民政府,贵州,三穗,556500 4. 贵州省黔南州独山县畜牧局,贵州,独山,558200 |
| 基金项目： | 国家茧丝发展风险基金 |
| 摘 要： | 贵州在自然地理气候、农村养蚕基础、桑树、家蚕种质资源、人力资源以及蚕茧品质等方面具有栽桑养蚕的优势条件,东桑西移在贵州的实施,对贵州蚕茧产业的发展带来了前所未有的发展机遇和动力.分析了贵州发展蚕业的优势,探讨了贵州蚕业发展的对策与措施. |
| 关 键 词： | 蚕业 东桑西移工程 发展机遇 对策措施 贵州 |
Diagnosis of Swine Edema Disease by the Establishment and Application of Polymerase Chain Reaction(PCR) |
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| YANG Li,WU Wei-heng,YANG Mao-sheng,JIA Hui-jun,YANG Sheng-kun,YANG Xiang-ce.Diagnosis of Swine Edema Disease by the Establishment and Application of Polymerase Chain Reaction(PCR)[J].Guizhou Agricultural Sciences,2009,37(6). | |
| Authors: | YANG Li WU Wei-heng YANG Mao-sheng JIA Hui-jun YANG Sheng-kun YANG Xiang-ce |
| Institution: | 1.Guizhou Animal Husbandry and Veterinary Medicine Institute;Guiyang;Guizhou 550005;2.Huaxi Town Veterinary Station of Guiyang Municipality;3.Bagongzai Township Government;Sanshui County;South-eastern Autonomy District of Guizhou;Sanshui;Guizhou 556500;4.Dushan Animal Husbandry Bureau;Southern Autonomy District of Guizhou;Dushan;Guizhou 558200;China |
| Abstract: | Polymerase chain reaction(PCR) Diagnosing is a common method for DNA rapid amplification and gene detection.This paper adopted the PCR to develop the diagnosis method for swine edema disease.Results showed that there was a band appeared when a 230 bp fragment of ED was amplified and the concentration of sensitivity detection for samples was 4.5 pg.The positive infectious rate was 75%(18/24),the infectious rates for HI was 54.17%(13/24),and the coincidence rate was 72.22%(13/18) for both methods.The study in... |
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