水稻白叶枯病菌在离体培养条件下生物膜形成的检测

分析了不同培养和测试条件(塑料和玻璃表面、培养时间、培养温度和培养基)对水稻白叶枯病菌(Xanthomonas oryzae pv. oryzae，Xoo)生物膜形成的影响，并测定了不同地区来源的Xoo野生型菌株、基因缺失突变体的生物膜形成。结果表明，在聚苯乙烯表面生长、用M210培养基在23 ℃下静止培养24 h是Xoo生物膜形成比较适宜的条件；不同地区来源的菌株生物膜形成能力不同；鞭毛相关基因fleQxoo、fliExoo和rbfCxoo以及H2O2降解调控基因oxyRxoo的缺失突变均影响生物膜形成。

Detection of biofilm formation of Xanthomonas oryzae pv.oryzae under static culture conditions in vitro

The effects of culture and detection conditions(e.g.plastic and glass surfaces,culture times,temperatures and media)on biofilm formation of Xanthomonas oryzae pv.oryzae(Xoo),and the biofilms of wild-type,gene deletion mutants of Xoo were assayed.The results indicated that the optimum culture conditions for biofilm formation of PX099A were shaking overnight in M210 medium,sub-cultured in M210 medium in 96-well polystyrene microplates,and incubated statically at 23℃ for 24 h.The biofilm formation of the wild-types Was different among the strains of different origins.The effects of deletion mutations of flagellar genes(fleQxoo,fliExoo and rbfCxoo)and H_2O_2-detoxification gene(oxyRxoo)on biofilm formation were found as well.