| 含CpG基序的PCV-2的ORF2基因真核表达载体的构建 |
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| 引用本文： | 曹帅,李俊,张祯涛,赵鸿雁,丛晓燕,张秀美,王金宝. 含CpG基序的PCV-2的ORF2基因真核表达载体的构建[J]. 动物医学进展, 2006, 27(10): 84-87 |
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| 作者姓名： | 曹帅 李俊 张祯涛 赵鸿雁 丛晓燕 张秀美 王金宝 |
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| 作者单位： | 莱阳农学院,山东青岛,266109;山东省农业科学院畜牧兽医研究所,山东济南,250100;山东省农业科学院畜牧兽医研究所,山东济南,250100;山东省畜牧兽医总站,山东济南,250100 |
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| 摘 要： | 通过PCR扩增得到PCV-2的ORF2基因,将其插入到了真核表达载体pVAX1中,构建了重组质粒pVAX1-ORF2。作为免疫佐剂,一段150 bp的人工合成CpG-ODN被插入到了pVAX1-OFR2的骨架结构中,构建了含有CpG-ODN的表达ORF2编码蛋白的重组质粒pVAX1-ORF2-CpG。
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| 关 键 词： | 猪圆环病毒2型 CpG-ODN 核酸疫苗 |
| 文章编号： | 1007-5038（2006）10-0084-04 |
| 收稿时间： | 2006-07-03 |
| 修稿时间： | 2006-07-03 |
Construction of Eukaryotic Vector Bearing ORF2 of PCV-2 and Containing CpG Motifs |
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| CAO Shuai,LI Jun,ZHANG Zhen-tao,ZHAO Hong-yan,CONG Xiao-yan,ZHANG Xiu-mei,WANG Jin-bao. Construction of Eukaryotic Vector Bearing ORF2 of PCV-2 and Containing CpG Motifs[J]. Progress In Veterinary Medicine, 2006, 27(10): 84-87 |
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| Authors: | CAO Shuai LI Jun ZHANG Zhen-tao ZHAO Hong-yan CONG Xiao-yan ZHANG Xiu-mei WANG Jin-bao |
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| Affiliation: | 1. Laiyang Agricutrual College, Qingdao, Shandong , 266109, China;2. Institute of Animal Science and Veterinary Medicine ,Shandang Academy of Agricultural Sciences, Jinan,Shandong , 250100, China, 3. Shandong General Station of Animal Husbandry and Veterinary Medicine ,Jinan, Shandong ,250100, China |
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| Abstract: | The subject of this study was to construct a recombinant plasmid DNA,which is based on ORF2 of PCV-2(porcine circovirus type 2) and contains a synthesized CpG-ODN of 150 bp in length.ORF2 of PCV-2 and a sequence consisting of 18 copies of three kinds of CpG motifs were induced into vector pVAX1's MCS and backbone structure,respectively.The recombinant plasmid is supposed to be used as a DNA vaccine in the further study. |
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| Keywords: | CpG-ODN |
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