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Analysis of nucleolar organizer regions by silver staining in two Lolium species
Authors:Valdemar Carnide  Juan Orellana
Affiliation:(1) Divisão de Gen~etica e Melhoramento de Plantas, IUTAD, Vila Real, Portugal;(2) Departamento de Gen~etica, Facultad de Biolog"itilde"a, Universidad Complutense, Madrid, Spain
Abstract:Summary The nucleolar organizer activity of diploid and tetraploid Lolium perenne L. and diploid Lolium multiflorumLam. varieties was analyzed by the silver staining procedure. Both species show six or twelve Ag-NORs (silver stained nucleolar organizer regions) at the diploid and tetraploid levels, respectively. A positive correlation between the size of secondary constrictions and Ag-NORs was observed.
Keywords:Lolium perenne  perennial ryegrass  Lolium multiflorum  Ag-nucleolar organizer regions  nucleolar activity
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