(1) Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, 243122, UP, India
Abstract:
The applicability of ribotyping based on 16S and 23S rRNA was evaluated for molecular epidemiological studies. Forty-eight
isolates of Pasteurella multocida isolated from different hosts and geographical locations and one reference isolate were ribotyped. Only four ribotypes were
found. All the isolates including reference isolate from wild carnivores had the same ribotype, though they had different
serotypes. The isolate from a tiger had one band in addition to the bands present in the major ribotype. The isolates from
lions represented two ribotypes; of these ribotypes, one (r2) had an additional band of 3.6 kbp, which was absent in all other
ribotypes. The second ribotype (r4) from a lion had one band missing (6 kbp) that was present in the other ribotypes. These
isolates were further typed using ERIC-PCR and REP-PCR. With ERIC-PCR and REP-PCR, higher D values of 0.83 and 0.89 were obtained. The current study revealed that ribotyping is not a very efficient typing tool for
use in molecular epidemiology for differentiation of isolates.