克隆包含以拟南芥为寄主的甘蓝黑腐病菌Xcc1067无毒基因的DNA片段

本研究探索了以拟南芥为寄主的甘蓝黑后病菌的致病条件，观察了其病症的发展．建立了菌株Ｘｃｃ１０６７的ｐＩＪ３２００为载体的基因文库，从基因文库中筛选出拟南芥Ｃｏｌｕｍｂｉａ为寄主的Ｘｃｃ１０６７菌株的无毒基因克隆，该克隆的进一步分析正在进行。

Cloning of DNA Fragment involved Avirulence Gene of Xanthomonas campestris pv. campestris Strain Xcc1067 in Arabidopsis thaliana (L.) Heynh

Understanding the molecular genetics basis of microbial plant disease is to require parallel study of both the pathogens and host plant. The crucifer Arabidopsis thaliana (L.) Heynh has many advantages as a model system for molecular biology, and it is a host of the pathogens Xanthomonas campestris pv. campestris. The methods of inoculation and symptoms developing were described in this paper. A genomic library of DNA of a X. c. pv.campestris strian Xcc1067 was constructed in pIJ3200, a broad host range cosmid vector within many Gram-negative hosts. The genomic libraries were screened by clones be individually conjugated into wild type strain Xcc8004 and transconjugants were screened for virulence to Columbia. One clone rendered Xcc8004 avirulent towards Columbia, but did not affect virulence to JI. 1 or Brassica campestris. The specificity of the affect for Columbia indicates that this clone contains an avirulence gene. Further work in the avirulence gene is now underway.