马铃薯茎尖脱毒培养影响因素研究

以马铃薯茎尖为外植体,通过组织培养研究了不同的茎尖大小、不同浓度和配比的细胞分裂素和生长素对脱毒效果的影响。结果表明:茎尖越小,脱毒率越高但成活率和成苗率越差;茎尖越大,成活率和成苗率高但脱毒率差。6种培养基中以MS+NAA 0.1 mg.L-1效果最好,成苗率为33.75%,为本试验中茎尖诱导分化成苗的最优培养基。

Influencing Factors of Potato Meristem Culture for Virus Elimination

The factors,including meristem size and concentration and proportioning of auxin and cytokinins in medium,were studied for efficacy of virus elimination.The smaller the meristem size,the higher the virus-free regenerated plantlet percentage,however,the survival percentage of inoculated meristem and percentage of regenerated plantlets were decreased.On the other side,the larger the meristem size,the higher the survival percentage of inoculated meristem and percentage of regenerated plantlets,but the efficacy of virus elimination was poor.Of six media tested the medium MS + NAA 0.1 mg.L-1 was the best medium for differentiation,with the percentage of regenerated plantlets being 33.75%.