| 鸡新城疫病毒B_(95)株的RT-PCR检测方法研究 |
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| 引用本文： | 申之义,苗儒,王俊平,焦静波,信洪一,李平安. 鸡新城疫病毒B_(95)株的RT-PCR检测方法研究[J]. 中国动物检疫, 2003, 0(4) |
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| 作者姓名： | 申之义 苗儒 王俊平 焦静波 信洪一 李平安 |
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| 作者单位： | 内蒙古农业大学 呼和浩特市010018(申之义，信洪一)，内蒙古动物防疫监督检验所 010051(苗儒，王俊平)，包头市动物防疫监督检验所 014040(焦静波)，内蒙古农业大学 呼和浩特市010018(李平安) |
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| 摘 要： | 本试验设计了一对B95 株的特异引物NDV -P1/P2,探讨了对其进行RT-PCR检测的可行性。结果证明 ,用该引物可以扩增出一条310bp的特异核酸带 ,敏感性为0.0043ng/ul。
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| 关 键 词： | NDVB95株 PCR检测 |
Studies on detection of NDV strain B_(95) by RT-PCR |
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| SHEN Zhi-yi MIAO Ru WANG Jun-ping JIAO Jing-bo XIN Hong-yi LI Ping-an. Studies on detection of NDV strain B_(95) by RT-PCR[J]. China Animal Health Inspection, 2003, 0(4) |
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| Authors: | SHEN Zhi-yi MIAO Ru WANG Jun-ping JIAO Jing-bo XIN Hong-yi LI Ping-an |
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| Abstract: | A pair of specific primers used in RT-PCR were designed according to the sequences of NDV B95 strain. The RT-PCR detection for NDV strain B95 was studied. Result showed that it was specific to strain B95 and the sensitivity is 0.0043ng/ul. |
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| Keywords: | NDV strain B95 PCR detection |
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