Germination and appressiorial formation by uredospores ofUromyces viciae-fabae exposed to inhibitors of polyamine biosynthesis |
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Authors: | Martina Reitz Dale Walters Bruno Moerschbacher |
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Affiliation: | (1) Institut fur Biologie III (Pflanzenphysiologie), RWTH Aachen, Germany;(2) Department of Plant Science, The Scottish Agricultural College, Nr Ayr KA6 5HW Auchincruive, UK |
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Abstract: | An examination was made of the effects of three polyamine biosynthesis inhibitors on germination and appressorium formation by uredospores of the bean rust fungusUromyces viciae-fabea on artificial membranes. The ornithine decarboxylase inhibitor -difluoromethylornithine had no effect on uredospore germination, even when used at 2mM, whereas appressorium formation was reduced by 63% at 0.5 mM and by 99% at 2mM. Methylglyoxal bis(guanylhydrazone), an inhibitor of S-andenosylmethionine decarboxylase, reduced germination when used at 0.025 mM, and at this concentration, appressorium formation was completed prevented. Uredospore germination was unaffected by as much as 3 mM cyclohexylamine, an inhibitor of spermidine esynthase, while appressorium formation was reduced at 1 mM and completely prevented at 3.3 mM. These results support previous suggestions that inhibitors of polyamine biosynthesis exert their main effect on the early stages of fungal development on the leaf surface.Abbreviantions CHA cyclohyxylamine - DFMO -difluoromethylornithine - MGBG methylglyoxal bis(guanylhydrazone) |
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Keywords: | Uromyces viciae-fabae uredospores germination appressorial formation polyamine biosynthesis inhibitors |
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