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Hemolytic activity of Trichomonas gallinae isolates does not correspond with clinical virulence
Authors:Richard W. Gerhold   Michael J. Yabsley  John R. Fischer
Affiliation:aSoutheastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA;bD.B. Warnell School of Forestry and Natural Resources, The University of Georgia, Athens, GA 30602, USA
Abstract:The hemolytic activity of 22 Trichomonas gallinae isolates was investigated using an 18 h erythrocyte hemolysis assay which has been shown to correlate with the clinical virulence of T. vaginalis. Absorbance of the assay supernatants was measured at 540 nm and expressed as percentage of complete hemolysis. Mean hemolytic activity of the T. gallinae isolates ranged from 3.5% to 53.4% and did not correspond with clinical virulence. The results of this investigation suggest hemolytic activity is not a useful in vitro virulence assay for T. gallinae.
Keywords:Avian erythrocytes   Hemolytic activity   Trichomonas gallinae   Virulence
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