| 乌珠穆沁羊FSHR基因第10外显子的PCR-SSCP检测及序列分析 |
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| 引用本文： | 黄丹丽,;杨章平,;任湘莲,;毛永江,;郭小雅,;瞿冬艳,;李云龙.乌珠穆沁羊FSHR基因第10外显子的PCR-SSCP检测及序列分析[J].家畜生态,2008(2):17-20. |
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| 作者姓名： | 黄丹丽 ;杨章平 ;任湘莲 ;毛永江 ;郭小雅 ;瞿冬艳 ;李云龙 |
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| 作者单位： | 扬州大学动物科学与技术学院 江苏扬州225009 |
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| 基金项目： | 江苏省自然科学基金（BK2003040）. |
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| 摘 要： | 利用PCR-SSCP技术检测乌珠穆沁羊123个样本的FSHR基因第10外显子的单核苷酸多态性（SNP）。结果未发现多态。测序后获得该羊FSHR基因第10外显子的核苷酸序列,通过DNA序列分析结果表明：绵羊FSHR基因第10外显子序列与小尾寒羊、牦牛、水牛和小鼠的同源性分别为100%、98%、98%和86%。提示：为FSHR基因第10外显子能否作为绵羊高繁殖力相关的侯选基因提供依据。
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| 关 键 词： | 绵羊 FSHR基因 多态性 PCR-SSCP |
PCR-SSCP Detection and DNA Sequence Analysis of Exon 10 of FSHR Gene in Three Sheep Populations |
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| HUANG Dan-li,YANG Zhang-ping,REN Xiang-Lian,MAO Yong-jiang,GUO Xiao-ya,QU Dong-yan,Li Yun-long.PCR-SSCP Detection and DNA Sequence Analysis of Exon 10 of FSHR Gene in Three Sheep Populations[J].Ecology of Domestic Animal,2008(2):17-20. |
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| Authors: | HUANG Dan-li YANG Zhang-ping REN Xiang-Lian MAO Yong-jiang GUO Xiao-ya QU Dong-yan Li Yun-long |
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| Institution: | HUANG Dan-li,YANG Zhang-ping*,REN Xiang-Lian,MAO Yong-jiang,GUO Xiao-ya,QU Dong-yan,Li Yun-long |
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| Abstract: | PCR-SSCP was applied to detect single nucleotide polymorphism （SNP） of exon 10 of the follicle-stimulating hormone receptor （FSHR） gene for 254 sheep, including Hu sheep, Ujumqin sheep and Tan sheep. The results demonstrated monopolymorphism. After sequenced, sheep FSHR gene exon10 DNA sequence was acquired. DNA sequence analysis showed that the homology of nucleotide sequence of exon 10 of FSHR gene among sheep, small-tailed Han sheep, yak, water buffalo and mouse were 100%. 98%,98% and 86%. The results implied that whether it provides evidence for the fact that the exon 10 of FSHR gene could be the candidate gene for high fecundity in sheep. |
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| Keywords: | sheep FSHR gene polymorphism PCR-SSCP |
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