| 巴马小型猪内源性反转录病毒感染HEK293细胞模型的建立 |
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| 引用本文： | 黄红梅,吕茂民,陈忠伟,王娜,赵武,陈凤莲,吴健敏,章金刚.巴马小型猪内源性反转录病毒感染HEK293细胞模型的建立[J].中国预防兽医学报,2006,28(3):312-315,365. |
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| 作者姓名： | 黄红梅 吕茂民 陈忠伟 王娜 赵武 陈凤莲 吴健敏 章金刚 |
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| 作者单位： | 1. 广西兽医研究所,广西,南宁,530001
2. 军事医学科学院,野战输血研究所,北京,100850 |
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| 基金项目： | 中国科学院资助项目;中国博士后科学基金;广西自然科学基金 |
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| 摘 要： | 首先将巴马小型猪（BM）、巴马香猪（BMXZ）外周血淋巴细胞分别与G418抗性HEK293细胞（G418^R293）共培养，通过G418加压筛选，除去共培养体系中巴马小型猪、巴马香猪外周血淋巴细胞，然后应用PCR及RT-PCR的方法对所制备的感染细胞模型进行系统鉴定。结果经6周共培养及3周加压筛选后，细胞的形态、生长速度及折光性均未见明显变化；PCR及RT-PCR鉴定表明，筛选后的共培养体系中已没有巴马小型猪、巴马香猪外周血淋巴细胞的存在；PERV特异性检测方法检测显示，该细胞模型的DNA中已有PERV的整合且有PERV特异性mRNA的表达。从而证实了猪外周血淋巴细胞来源的PERV在体外也能够感染人源细胞系，为研究PERV的生物学特性及病原安全性的评价搭建了技术平台。
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| 关 键 词： | G418抗性 HEK293细胞 猪内源性反转录病毒 |
| 文章编号： | 1008-0589（2006）03-0312-04 |
| 收稿时间： | 2005-02-19 |
| 修稿时间： | 2005-02-19 |
Establishment of model for HEK293 cell line infected by BM porcine endogenous retrovirus |
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| HUANG Hong-mei,LV Mao-min,CHEN Zhong-wei,WANG Na,ZHAO Wu,CHEN Feng-lian,WU Jian-min,ZHANG Jin-gang.Establishment of model for HEK293 cell line infected by BM porcine endogenous retrovirus[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(3):312-315,365. |
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| Authors: | HUANG Hong-mei LV Mao-min CHEN Zhong-wei WANG Na ZHAO Wu CHEN Feng-lian WU Jian-min ZHANG Jin-gang |
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| Institution: | 1. Guangxi Veterinary Institute, Nanning 530001, China; 2. Institute of Transfusion Medicine, The Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract: | Based on HEK293 cell strains with G418 resistance, we co-cultured the cells and PBL of BM minipigs and screened by G418. Systematic evaluation was executed on the cell models by PCR and RT-PCR after removing of PBL. We constructed HEK293 cell models infected by BM PERV, and verified the infectivity of PERV from porcine PBL to human-derived cells in vitro, which helped in PERV biological characteristics and pathogen safety research. |
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| Keywords: | G418 resistance HEK293 cell porcine endogenous retrovirus |
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