Ovine Metabolism of Saponins: Evaluation of a Method for Estimating the Ovine Uptake of Steroidal Saponins from Narthecium ossifragum |
| |
| Authors: | Flåøyen A Wilkins AL Deng D Brekke T |
| |
| Institution: | (1) National Veterinary Institute, PO Box 8156 Dep., N-0033 Oslo, Norway;(2) Department of Large Animal Clinical Sciences, Norwegian School of Veterinary Science, PO Box 8146 Dep., N-0033 Oslo, Norway;(3) Chemistry Department, School of Science and Technology, University of Waikato, Private Bag 3105, Hamilton, New Zealand;(4) National Veterinary Institute, PO Box 8156 Dep., N-0033 Oslo, Norway |
| |
| Abstract: | A sheep was dosed three times per day over six consecutive days with 70 g Narthecium ossifragum, and once on the seventh day with 70 g N. ossifragum. Additionally, it was dosed once on days 1–7 with 20 mg of 20,23,23-2H3]sarsasapogenin. After 7 days, the sheep was killed and GC-MS analysis of the free and conjugated sapogenin content in bile, urine, rumen, duodenum, jejunum, colon and rectum samples collected from the sheep, faecal samples collected on days 4–7, and dosed plant material was performed. The N. ossifragum contained mainly sarsasapogenin and smilagenin. Only negligible levels of deuterium-labelled sarsasapogenins were detected in the samples from the animal. Ingested saponins were quickly hydrolysed in the rumen to free sapogenins and, in part, epimerized at C-3 to afford episapogenins. The absorption of free sapogenins appeared to occur in the jejunum. The concentration of sapogenins in faeces reached a plateau 108 h after dosing started. |
| |
| Keywords: | episarsasapogenin liver metabolism Narthecium ossifragum saponin sarsasapogenin sheep smilagenin |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
