Identification of immunodominant antigens of Mycobacterium bovis by expression library immunization |
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Authors: | Beltrán Paulina K Gutiérrez-Ortega Abel Puebla-Pérez Ana M Gutiérrez-Pabello José A Flores-Valdez Mario A Hernández-Gutiérrez Rodolfo Martínez-Velázquez Moisés Alvarez Angel H |
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Institution: | Centro de Investigación y Asistencia en Tecnología y dise?o del Estado de Jalisco, Guadalajara, Jalisco, Mexico. |
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Abstract: | This study combines two methodologies - vector expression of a genomic library and proteomics - to identify immunogenic proteins of Mycobacterium bovis. Immunization of BALB/c mice with a plasmid DNA pool from the library, containing approximately 8000 clones, induced a humoral response that facilitated the detection of 12 antigenic proteins by Western blotting. Two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry identified four proteins (Cpn60-1, HSP70, EF-Tu, and AdoHcyase). Such genomic immunization offers the possibility of in vivo screening of potential candidate M. bovis antigens. |
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