不同诱变方法对抗咪唑乙烟酸水稻筛选的影响

为筛选抗咪唑乙烟酸水稻,进行了水稻种子敏感性检测以确定筛选浓度,应用紫外线、叠氮化钠及复合诱变对种子进行诱变筛选.结果表明:抗性筛选的适宜药剂浓度为0.3mL·L~(-1);在此浓度下,经75W紫外灯照射1,2,3h的种子未筛选出抗性植株;种子经不同浓度、时间NaN_3处理后筛选出抗性植株,以NaN_3诱变剂量为8mmol·h·L~(-1)(4mmol·L~(-1)×2h)抗性比率最高,出苗率为7.14%;相比NaN_3诱变,复合诱变的种子并没有显著不同.将筛选出的植株苗期喷5mL·L~(-1)眯唑乙烟酸,筛选出4株对眯唑乙烟酸有抗性的水稻植株.

Selection of Imazethapyr-resistant Rice with Mutation

Rice seed sensitivity bioassay was tested to determine the suitable concentration of selection, and the rice seeds were mutagenized with ultraviolet rays or NaN_3 in the selection of imazethapyr-resistant rice. The result showed the suitable concentration of herbicide selection was 0.3mL·L~(-1). The resistant plantlets were not obtained in the seeds mutagenized with ultraviolet rays (75W), while those were obtained in the seeds mutagenized with NaN_3 at different concentrations and induced time. The resistant rate was the greatest on the 8mmol·h·L~(-1) mutant dose (4mmol·L~(-1)×2h), in which the percent of the seedling was 7.14%. Seeds mutagenized with NaN_3 were not significantly different with those mutagenized with ultraviolet rays and NAN_3. The selected seedlings were sprayed with 5mL·L~(-1) imazethapyr, and 4 imazethapyr-resistant rice plants were obtained.