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Identification by transfer blot of antigens reactive in the enzyme-linked immunosorbent assay (ELISA) in rabbits immunized and a calf infected with Cryptosporidium sp |
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| Authors: | A Lazo O O Barriga D R Redman S Bech-Nielsen |
| Affiliation: | 1. MTA–SZTE Analysis and Stochastic Research Group, University of Szeged, Aradi vértanúk tere 1, Szeged 6720, Hungary;2. Bolyai Institute, University of Szeged, Aradi vértanúk tere 1, Szeged 6720, Hungary;1. Department of Mathematics, Institut Teknologi Bandung, Bandung 40132, Indonesia;2. School of Mathematics, Statistics and Computer Science, University of KwaZulu-Natal, Durban 4041, South Africa;3. Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia;4. Department of Mathematics, Diponegoro University, Semarang 50275, Indonesia |
| Abstract: | Soluble and particulate fractions of Cryptosporidium sp. oocysts from cattle were obtained by homogenization and sonication. Electrophoresis of the soluble fraction in polyacrylamide gels with sodium dodecyl sulfate and silver staining revealed the presence of 41 bands. Enzyme-linked immunosorbent assay (ELISA) of sera from rabbits immunized with either fraction and from a calf 40 days after infection showed that the animals produced specific antibodies. Enzyme-linked immunoelectrotransfer blot tests revealed the presence of five antigens with the rabbit sera and nine with the calf serum. ELISA proved to be an appropriate test for diagnosis of cryptosporidiosis. Selection of reactive antigens may improve the quality of diagnosis and/or reveal the presence of protective materials in the parasite. |
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