蓖麻蚕不同组织器官中胰蛋白酶抑制剂的种类与分析

胰蛋白酶抑制剂(TI)是鳞翅目昆虫体内广泛存在的一种蛋白酶抑制剂,主要功能是抑制胰蛋白酶的降解。采用非变性聚丙烯酰胺凝胶电泳和胰蛋白酶抑制剂活性染色,对18个蓖麻蚕品种5龄第5天的血液及其它组织中TI种类的分布进行了调查。经碱性聚丙烯酰胺凝胶电泳,在血液中共检测到8种TIs,分别命名为ETI-1~ETI-8,品种间存在多态性分布;在头和脂肪体中分别检测到1种TI;在体壁中检测到3种TIs。经酸性聚丙烯酰胺凝胶电泳,在血液中共检测到4种TIs,分别命名为ETI-b1、ETI-b2、ETI-b3和ETI-b4,品种间种类分布无差异;在血液中检测到的TI包含了在头、脂肪体和体壁中检测到的TI种类。

Investigation of Trypsin Inhibitor Types From Different Tissues of the Eri-silkworm,Philosamia cynthia ricini

Trypsin inhibitors(TIs) present widely in bodies of the Lepidoptera insects,which play important roles in inhibiting proteolysis of trypsin.By using method of native-polyacrylamide gel electrophoresis followed by active staining,we investigated the polymorphism of TIs in hemolymph and major tissues at 5th d of 5th instar of 18 varieties of eri-silkworm,Philosamia cynthia ricini.In the result of the alkaline native-polyacrylamide gel electrophoresis,there are 8 TIs in hemolymph,named as ETI-1～ETI-8,and a little difference among different varieties.Only 1 TI was detected in head and fat body,respectively,while 3 TIs were detected in body wall.In the result of the acidity native-polyacrylamide gel electrophoresis,4 TIs were detected in hemolymph,which were named as ETI-b1,ETI-b2,ETI-b3,and ETI-b4,respectively.The distribution of TIs in hemolymph among 18 eri-silkworm varieties was same.TIs in hemolymph included TIs detected in head,fat body,and body wall.