| 漆酶高产菌株筛选及漆酶基因片段的克隆 |
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| 引用本文： | 任广明,李滇华,曲娟娟,于学佳,王朋. 漆酶高产菌株筛选及漆酶基因片段的克隆[J]. 中国林副特产, 2010, 0(5): 39-41 |
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| 作者姓名： | 任广明 李滇华 曲娟娟 于学佳 王朋 |
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| 作者单位： | 1. 伊春林科院原生态食用菌研究所,黑龙江,伊春,153000
2. 东北农业大学资源与环境学院,黑龙江,哈尔滨,150030 |
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| 摘 要： | 研究利用不同底物浓度、不同培养时间、不同培养基为优化因素,对漆酶产生菌株筛选条件进行了初步研究,从广泛4株食用真菌中筛选具有高产漆酶特性的真菌,双孢菇2796,并以双孢菇2796为供体菌,提取菌株RNA,运用RT-PCR方法对漆酶基因进行扩增,克隆后得到长度为160bp的漆酶基因片段。
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| 关 键 词： | 漆酶 RT-PCR 双孢菇2796 |
The Rearch of Screening of Fugal Laccase Strain and Cloning of Laccase Gene Fragments |
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| Ren Guangming,Li Dianhua,Qu Juanjuan,Yu Xuejia,Wang Peng. The Rearch of Screening of Fugal Laccase Strain and Cloning of Laccase Gene Fragments[J]. Forest By-product and Speciality in China, 2010, 0(5): 39-41 |
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| Authors: | Ren Guangming Li Dianhua Qu Juanjuan Yu Xuejia Wang Peng |
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| Affiliation: | 1.College of Forestryource and science,yichun,153000;2.College of Resource and Environmental science,Northeast Agricultural University,Harbin,150030) |
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| Abstract: | Laccase production strain incubation conditions were studied by different substrate concentrations and different incubation time,different medium.Agaricus bisporus 2796 as the best Laccase production strain was selected from a wide range of consumption of 14 fungal,The extraction of total RNA from Agaricus bisporus 2796 and RT-PCR technology was used to gain a length of 160bp fragment of Laccase cDNA sequences. |
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| Keywords: | RT-PCR |
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