| 棉花基因枪茎尖转化研究 |
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| 引用本文： | 刘传亮,于娅,王玉芬,耿立召,李付广.棉花基因枪茎尖转化研究[J].棉花学报,2003,15(6):361-366. |
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| 作者姓名： | 刘传亮 于娅 王玉芬 耿立召 李付广 |
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| 作者单位： | 1. 中国农业科学院棉花研究所,农业部棉花遗传改良重点开放实验室,河南,安阳,455004
2. 河北农业大学,河北,保定,071000 |
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| 基金项目： | 863计划及国家转基因植物研究专项资助(J2002-B-001) |
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| 摘 要： | 对棉花茎尖轰击转化的多种因素进行了分析,建立了较为完善的基因枪茎尖转化体系,使抗性转化率达到4.7％～9.4％,转化周期缩短至2～3个月,改进了抗性筛选程序,建立了4次筛选法,获得91株抗生素抗性植株;同时对部分植株目的基因的表达量进行了初步的遗传分析。结果表明,茎尖的轰击状态与抗性筛选程序对转化周期与转化率具有决定性作用。
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| 关 键 词： | 棉花 基因枪 遗传转化 抗性筛选程序 |
| 文章编号： | 1000-7807(2003)06-0361-06 |
| 修稿时间： | 2003年2月17日 |
Study on Transformation of Meristem via Gene-gun Bombardment |
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| LIU Chuan-liang,YU Ya,WANG Yu-fen,GENG Li-zhao,LI Fu-guang.Study on Transformation of Meristem via Gene-gun Bombardment[J].Cotton Science,2003,15(6):361-366. |
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| Authors: | LIU Chuan-liang YU Ya WANG Yu-fen GENG Li-zhao LI Fu-guang |
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| Abstract: | An improved transformation system of meristem via gene-gun bombardment was established after studies on many influential factors. The transformation rate of anti-antibiotic reached 4. 7% -9. 4% and the transformation cycle decreased to 2-3 months; Four-times Selective Strategy was founded after improving selection of transformation plants; 91 anti-antibiotic transgenic plants were obtained, some of them had been carried out their Bt measurements in leaves and analyses of their genetic rules. All the results showed the state of the shoot-tips and the Selective Strategy acted vital roles on transformation rate and cycle. |
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| Keywords: | cotton gene-gun transformation se- lective strategy |
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