部分小麦农家品种抗白粉病基因推导与遗传多样性分析

为给小麦农家品种抗白病遗传研究及育种应用提供依据,采用21个小麦白粉茵已知毒性茵株和30个鉴别寄主对18个小麦农家品种进行苗期接种鉴定.对品种所携带的抗白粉病基因进行推导;选取11对与7B染色体上抗白粉病基因紧密连锁的SSR标记,对小麦农家品种抗白粉病基因遗传多样性进行分析.结果表明.除小白冬麦和唐麦4号、白蚰蜒条和芒麦-1抗谱相同外,其余农家品种抗谱都不尽相同.微卫星引物PIC值最高为0.78,最低0.19,平均0.61.根据各品种的指纹图谱,利用UPGMA方法进行聚类分析,品种间的遗传相似系数范围为0.72～1.OO,所有参试品种在遗传相似系数0.735处被划分为3类,其中,蚂蚱麦和笨三月黄,白蚰蜒条、有芒瞎八斗、芒麦-1、小白冬麦和鉴别寄主复壮30.蝼蛄腚、霸王鞭和红秃头带型完全一致,遗传相似系数为1.00.由此可见,小麦农家品种中抗白粉病基因存在着较为丰富的等位变异,在7B染色体长臂末端区域上可能存在更多的Pm5复等位基因或与其紧密连锁的抗白粉病基因簇.

Postulation of Powdery Mildew Resistant Genes Carried in Some Chinese Wheat Landraces and the Genetic Diversity Analysis

Eighteen Chinese wheat landraces were inoculated at seedling stage with 21 pathotypes of wheat powdery mildew(Blumeria graminis f.sp.tritici),respectively,and the possible resistant gene(s) carried was postulated.The genetic diversity of powdery mildew resistant genes was investigated among these Chinese wheat landraces using 11 wheat SSR molecular markers.The results indicated that the spectra of Xiaobadongmai and Tangmai 4,Baiyouyantiao and Mangmai 1 were the same while the remaining ones were all differe...