| 耕层土壤镰刀菌单胞系的建立和基因组DNA的快速抽提 |
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| 引用本文： | 李志岗,宋东辉,王建明,贺运春,李志远. 耕层土壤镰刀菌单胞系的建立和基因组DNA的快速抽提[J]. 山西农业大学学报(自然科学版), 2002, 22(1): 32-35 |
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| 作者姓名： | 李志岗 宋东辉 王建明 贺运春 李志远 |
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| 作者单位： | 1. 山西农业大学农学院,山西太谷030801
2. 大同师范学院生物科学系,山西大同039031 |
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| 基金项目： | 山西省青年基金资助项目 (9910 3 8) |
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| 摘 要： | 采用改进的镰刀菌选择性培养基和产孢培养基对采自大田、苗圃耕层土壤中的镰刀菌进行单孢分离 ,共分离出 5 0个镰刀菌菌株 ,而且改进的镰刀菌选择性培养基对目标菌选择性强 ,产孢量大。用改进的SDS -SEAVG分离法和快速抽提法提取镰刀菌基因组DNA ,结果显示 ,用改进的镰刀菌DNA抽提方法抽提的DNA分子量大于 5 0kb ;用快速抽提方法获得的镰刀菌单孢系DNA可用于PCR扩增。
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| 关 键 词： | 遗传多样性 镰刀菌 单孢系 基因组DNA |
| 文章编号： | 1671-8151(2002)01-0032-04 |
| 修稿时间： | 2000-05-20 |
Establishment of Single Conidial Strains of Fusarium and Rapid Isolation Their Genomic DNA |
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| LI Zhi|gang et al.. Establishment of Single Conidial Strains of Fusarium and Rapid Isolation Their Genomic DNA[J]. Journal of Shanxi Agricultural University(Nature Science Edition), 2002, 22(1): 32-35 |
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| Authors: | LI Zhi|gang et al. |
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| Abstract: | Fifty isolates of% Fusarium% were recovered from topsoil of crop field and nursing bed with the selective media which were improved. The results showed that the selective media for isolating %Fusarium% were not contaminated, and the media for producing conidiospore have produced a bigger yield. Genomic DNA were extracted from the single conidial strains of %Fusarium% using improved SDS-SEAVG method and rapid method respectively. Agarose elecphresis demonstrated that the molecular weight of DNA which were extracted from the single conidial strains %Fusarium% using improved method was more than 50kb. Most of the obtained DNA using the rapid method were suitable for PCR amplification. |
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| Keywords: | Genetic diversity %Fusarium% Single conidial Strain genomic DNA |
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