| 紫外辐射诱导草鱼肾脏细胞系（CIK）的程序性死亡 |
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| 引用本文： | 屈三甫,郑从义,胡国斌,彭芳,栾亚华,丁桂珍,刘颖.紫外辐射诱导草鱼肾脏细胞系（CIK）的程序性死亡[J].水产学杂志,1999,12(1):42-46. |
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| 作者姓名： | 屈三甫 郑从义 胡国斌 彭芳 栾亚华 丁桂珍 刘颖 |
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| 作者单位： | [1]武汉大学生命科学学院，武汉430072 [2]武汉市水产科学研究所，武汉430065 |
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| 摘 要： | 本文报道研究紫外辐射对离体培养的草鱼肾脏细胞系(CIK)的影响。实验结果显示，在距离15W紫外灯40cm下照射草鱼的CIK细胞3min后，CIK细胞核即产生固缩、碎裂现象，琼脂糖凝胶电泳可见典型的DNA梯形带，流式细胞仪检测发现有亚G1峰产生。结果表明，短时间的紫外辐射即可诱导CIK细胞发生细胞程序性死亡。
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| 关 键 词： | CIK细胞 肾脏细胞 诱导 流式细胞仪检测 琼脂糖凝胶电泳 照射 程序性死亡 草鱼 离体培养 紫外辐射 |
PROGRAMMED CELL DEATH INDUCED BY ULTRAVIOLET IRRADIATION IN THE CELL LINES OF
KIDNEY FROM GRASS CARP |
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| Qu Sanfu Zheng Congyi Hu Gaobi Peng Fang Luan Yahua.PROGRAMMED CELL DEATH INDUCED BY ULTRAVIOLET IRRADIATION IN THE CELL LINES OF
KIDNEY FROM GRASS CARP[J].Chinese Journal of Fisheries,1999,12(1):42-46. |
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| Authors: | Qu Sanfu Zheng Congyi Hu Gaobi Peng Fang Luan Yahua |
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| Abstract: | S The effects of ultraviolet
irraddiation on cultured Grass carp kindney cell lines (CIK) were investigated in this article.The
results revealed that after exposed to a 15 W ultraviolet lamp at a distance of 35cm for 1-3min,
CIK cells went through nuclear condensation and fragmentation;In addition,agarose gel
electrophoresis of DNA extracted from irradiated CIK cells revealed typical thatladderbands and
Sub-Gl pesk in irradiated cells could be detected by flow cytometric method.These results
indicated that ultraviolet irraddiation can induce programmed cell death in CIK cells. |
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| Keywords: | Ultraviolet irraddiation Grass carp kindney cell lines Programmed cell death |
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