复方中药秦公散对小鼠脾脏和外周血中T、B淋巴细胞的影响

为探讨治疗奶牛乳房炎的复方中药秦公散对小鼠脾脏和外周血中T淋巴细胞和B淋巴细胞亚群的影响，采用流式细胞仪测定各处理组小鼠脾脏和外周血中用CD3+、CD4+ 、CD8+标记的T淋巴细胞百分率及CD4+/CD8+的比值和CD19+标记的B淋巴细胞百分率。结果表明，与蒸馏水组相比，秦公散高剂量组小鼠脾脏和外周血中CD3+、CD4+细胞百分率和CD4+/CD8+的比值均显著提高，对环孢菌素（CsA）抑制小鼠有显著恢复作用(P<0.05)；秦公散高剂量（20 g/kg体重）可显著提高健康小鼠脾脏和外周血中CD19+细胞百分率(P<0.05),对环孢菌素抑制小鼠的CD19+细胞百分率有显著恢复作用(P<0.05)。结果表明，治疗奶牛乳房炎的复方中药秦公散通过提高机体中CD3+细胞、CD19+细胞百分率和调节CD4+/CD8+之间的平衡来显著提高小鼠机体的细胞免疫功能和恢复由环孢菌素（CsA）抑制的小鼠机体免疫力。

The Influence of Chinese Medicine Qingongsan on the T,B Lymphocyte of Spleen and Peripheral Blood in Mice

To observe the influence of Chinese medicine Qingongsan on the T,B lymphocyte of spleen and peripheral blood in mice, the flow cytometry method were adopted to determine the percentage of CD3+〖KG-*2〗, CD8+，CD4+ and CD19+ lymphocytes and CD4 +/CD8 + value. Chinese medicine Qingongsan signifcantly enhanced the percentage of CD3+, CD4+ and CD19+ lymphocytes and CD4 +/CD8 + value in peripheral blood and recovered the percentage of CD3+,CD3+CD８+and CD19+ lymphocytes in mice inhibited by cyclosporin. The results showed that Chinese medicine Qingongsan can significantly enhance the immune function and recover the immune function in mice inhibited by cyclosinfluence.