Mapping of Lr56 translocation recombinants in wheat |
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| Authors: | Mohamed Somo Seyed Mostafa Pirseyedi Xiwen Cai Roshan Sharma Poudel Shiaoman Chao Francois Marais |
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| Institution: | 1. Department of Plant Sciences, North Dakota State University, Fargo, ND, USA;2. Department of Plant Pathology, North Dakota State University, Fargo, ND, USA;3. US Department of Agriculture, Agricultural Research Service Biosciences Research Laboratory, Fargo, ND, USA |
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| Abstract: | In an attempt to transfer the Lr56/Yr38 resistance loci from Aegilops sharonensis to wheat, a 6A‐6Ssh chromosome translocation was produced. It involves essentially the entire chromosome 6Ssh with a small terminal segment of 6AL. Induced homoeologous recombination of the translocated chromosome with 6A produced numerous recombinants including three recombined chromosomes carrying Lr56 that could not be precisely mapped for lack of suitable markers. This study aimed to determine the chromosomal locations of the translocation breakpoints in these three recombinants using various DNA markers as well as physical and genetic mapping. The three recombinants Lr56‐39, ‐157 and ‐175 carry small segments of Ae. sharonensis chromatin distally to the Xgpw4329 and IWA5416 loci near the 6AS telomere. The Ae. sharonensis chromatin that remains in each line includes a homoeolocus of the wheat marker locus Xdupw217 (on 6BS) and its characteristic amplification product can be used as a dominant marker for the presence of Lr56. Of the three recombined chromosomes, Lr56‐157 retained the least alien chromatin and appears to be the best candidate for use in wheat breeding. |
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| Keywords: | gene transfer leaf rust resistance breeding wide hybridization |
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