ELISA indexing of commercial carnations for carnation mottle virus using a urease-antibody conjugate |
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| Authors: | JANE R MORAN JENNY M WILSON R G GARRETT P R SMITH |
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| Institution: | Plant Research Institute, Department of Agriculture, Burnley. Victoria 3121. Australia |
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| Abstract: | The high sensitivity of the urease form of enzyme-linked immunosorbent assay (ELISA) system allowed the use of pooled samples for the detection of carnation mottle virus (CarMV) with greater sensitivity than the phosphatase system. The urease-ELISA was used for a field assessment of the effectiveness of a Plant Improvement Programme (PIP) for carnations operated by the Victorian Department of Agriculture for the control of carnation mottle virus. On properties growing only PIP carnations, infection with CarMV averaged 0.95%. whereas cm properties growing carnations from ordinary commercial sources, all plants sampled contained the virus. The Plant Improvement Programme provided effective control of CarMV in Sim carnations. |
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