Gamma-A cold agglutinin: importance of disulfide bonds in activity and structure |
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Authors: | B R Andersen |
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Abstract: | Cleavage of disulfide bonds caused by mild reduction with mercaptoethanol produces a reversible loss of cold agglutinin activity and antigen-binding ability in a gamma-A polymer cold agglutinin. Return of agglutination and antigen-binding ability occur only in those molecules that have not been depolyimerized by reduction. Loss of activity becomes irreversible if reduction is followed immediately by alkylation. |
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